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K. Umasankar ¹, Balwin Nambikkairaj ¹, D. Manley Backyavathy ¹

Affiliations
1 Department of Zoology, Voorhees College, Vellore-632 001, T. N., IN

Abstract

A common complication of diabetes is impaired wound healing. Systemic Rosmarinus officinalis oil improves healing in diabetics, which is dose dependent and may have side effects. There is very less information regarding topical Rosmarinus officinalis oil use. The objective of this study was to evaluate the effects of topical Rosmarinus officinalis oil on wound healing. Diabetes was induced in Wistar rats by using streptozotocin. The control group comprised age-matched animals not submitted to streptozotocin injection. Diabetic state was confirmed by glycosuria and hyperglycemia. Under tribromoethanol anaesthesia, four skin wounds (4mm diameter) were performed on shaved dorsal area (2 each side of median line). Topical Rosmarinus officinalis oil was applied daily only on both wounds on right side of median line. Animals were sacrificed on day 3 and 7 after surgery and tissue samples were prepared and observed under light microscopy. Histological, histometric and stereological methods were used for analysis. Topical Rosmarinus officinalis oil accelerated wound closure in diabetic and non-diabetic rats and the results were found to be more active than antibiotic treated controls. Topical Rosmarinus officinalis oil could be helpful in diabetics in order to improve the wound healing process avoiding possible adverse effects from systemic medication. All the values were statistically significant.

Keywords

Streptozotocin, Wound Healing, Rosmarinus officinalis Oil, Wistar Rats.

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P. N. Sudha ¹, D. Manley Backyavathy ², K. Sudhakar ³

Affiliations
1 Department of Chemistry, D. K. M. College for Women, Vellore-632 001, T.N., IN
2 Department of Zoology, Voorhees College, Vellore-632 001, T.N., IN
3 Department of Biological Sciences, Chezhian College of Education, Polur-606 803, T.N., IN

Abstract

Malathion, a pesticide in the organophosphate chemical family is the most commonly used pesticide in our country. Symptoms of exposure to malathion include headache, nausea, vomiting, burning in eyes, difficulty in breathing and lethargy. Earthworms have been used as biomarkers for assessing chemical environmental pollution. Due to their low cost, easy handling, their direct body contact with the soil and ethical issues related to mammalian studies, earthworms were chosen for the present study. Three doses of malathion 150, 300 and 600 mg/kg soil were used as treated soil. The effect of application of malathion on the earthworm Lampito mauritii was studied for 5, 10, 15 and 30 days exposure by evaluating the body weight, number of cocoons laid and the hatching efficiency of the cocoons. The results showed that there is a direct dose response relationship with respect to both, the concentration and time of application of malathion. The results have been statistically analysed.

Keywords

Pesticides, Melathion, Earthworm, Lampito mauritii, Non-Target Organism, Cocoons, Biomarkers.

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